!Series_title	"Transcriptomic Characterization of neuroblastoma by Single Cell RNA Sequencing"
!Series_geo_accession	"GSE137804"
!Series_status	"Public on Sep 18 2020"
!Series_submission_date	"Sep 20 2019"
!Series_last_update_date	"Sep 21 2020"
!Series_pubmed_id	"32946775"
!Series_web_link	"https://www.cell.com/cancer-cell/pdf/S1535-6108(20)30425-6.pdf"
!Series_summary	"Neuroblastoma (NB) is the most common malignant solid tumor in the abdomen of children, with high degree of malignancy, poor prognosis and unknown pathogenesis. So we aim to investigate intra-tumor heterogeneity of neuroblastoma at single cell resolution."
!Series_overall_design	"Single cell mRNA profiles of sixteen primary tumors from sixteen patients with neuroblastoma, four adrenal tissue and two embryos from six aborted fetus were generated by 10X Genomics plartform, without technical replication, and sequenced by NovaSeq 6000 (Illumina)"
!Series_type	"Expression profiling by high throughput sequencing"
!Series_contributor	"Rui,,Dong"
!Series_contributor	"Jia,,Wang"
!Series_contributor	"Kai,,Li"
!Series_contributor	"Kuiran,,Dong"
!Series_contributor	"Yong,,Zhan"
!Series_contributor	"Chunjing,,Ye"
!Series_sample_id	"GSM4088774 GSM4088775 GSM4088776 GSM4088777 GSM4088778 GSM4088779 GSM4088780 GSM4088781 GSM4088782 GSM4088783 GSM4088785 GSM4088786 GSM4088787 GSM4088788 GSM4654667 GSM4654668 GSM4654669 GSM4654670 GSM4654671 GSM4654672 GSM4654673 GSM4654674 "
!Series_contact_name	"rui,,Dong"
!Series_contact_email	"rdong@fudan.edu.cn"
!Series_contact_institute	"Department of Pediatric Surgery, Children’s Hospital of Fudan University, and Shanghai Key Laboratory of Birth Defect"
!Series_contact_address	"399 Wan Yuan Road"
!Series_contact_city	"Shanghai"
!Series_contact_zip/postal_code	"201102"
!Series_contact_country	"China"
!Series_supplementary_file	"ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE137nnn/GSE137804/suppl/GSE137804_RAW.tar"
!Series_platform_id	"GPL24676"
!Series_platform_taxid	"9606"
!Series_sample_taxid	"9606"
!Series_relation	"BioProject: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA573097"
!Series_relation	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRP222837"

!Sample_title	"T10"	"T19"	"T27"	"T34"	"T40"	"T44"	"T69"	"T71"	"T75"	"T92"	"F2"	"F7"	"F106"	"F107"	"F366"	"F379"	"T162"	"T175"	"T188"	"T200"	"T214"	"T230"
!Sample_geo_accession	"GSM4088774"	"GSM4088775"	"GSM4088776"	"GSM4088777"	"GSM4088778"	"GSM4088779"	"GSM4088780"	"GSM4088781"	"GSM4088782"	"GSM4088783"	"GSM4088785"	"GSM4088786"	"GSM4088787"	"GSM4088788"	"GSM4654667"	"GSM4654668"	"GSM4654669"	"GSM4654670"	"GSM4654671"	"GSM4654672"	"GSM4654673"	"GSM4654674"
!Sample_status	"Public on Sep 18 2020"	"Public on Sep 18 2020"	"Public on Sep 18 2020"	"Public on Sep 18 2020"	"Public on Sep 18 2020"	"Public on Sep 18 2020"	"Public on Sep 18 2020"	"Public on Sep 18 2020"	"Public on Sep 18 2020"	"Public on Sep 18 2020"	"Public on Sep 18 2020"	"Public on Sep 18 2020"	"Public on Sep 18 2020"	"Public on Sep 18 2020"	"Public on Sep 18 2020"	"Public on Sep 18 2020"	"Public on Sep 18 2020"	"Public on Sep 18 2020"	"Public on Sep 18 2020"	"Public on Sep 18 2020"	"Public on Sep 18 2020"	"Public on Sep 18 2020"
!Sample_submission_date	"Sep 20 2019"	"Sep 20 2019"	"Sep 20 2019"	"Sep 20 2019"	"Sep 20 2019"	"Sep 20 2019"	"Sep 20 2019"	"Sep 20 2019"	"Sep 20 2019"	"Sep 20 2019"	"Sep 20 2019"	"Sep 20 2019"	"Sep 20 2019"	"Sep 20 2019"	"Jul 05 2020"	"Jul 05 2020"	"Jul 05 2020"	"Jul 05 2020"	"Jul 05 2020"	"Jul 05 2020"	"Jul 05 2020"	"Jul 05 2020"
!Sample_last_update_date	"Sep 18 2020"	"Sep 18 2020"	"Sep 18 2020"	"Sep 18 2020"	"Sep 18 2020"	"Sep 18 2020"	"Sep 18 2020"	"Sep 18 2020"	"Sep 18 2020"	"Sep 18 2020"	"Sep 18 2020"	"Sep 18 2020"	"Sep 18 2020"	"Sep 18 2020"	"Sep 18 2020"	"Sep 18 2020"	"Sep 18 2020"	"Sep 18 2020"	"Sep 18 2020"	"Sep 18 2020"	"Sep 18 2020"	"Sep 18 2020"
!Sample_type	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"	"SRA"
!Sample_channel_count	"1"	"1"	"1"	"1"	"1"	"1"	"1"	"1"	"1"	"1"	"1"	"1"	"1"	"1"	"1"	"1"	"1"	"1"	"1"	"1"	"1"	"1"
!Sample_source_name_ch1	"neuroblastoma tissue"	"neuroblastoma tissue"	"neuroblastoma tissue"	"neuroblastoma tissue"	"neuroblastoma tissue"	"neuroblastoma tissue"	"neuroblastoma tissue"	"neuroblastoma tissue"	"neuroblastoma tissue"	"neuroblastoma tissue"	"fetal adrenal tissue"	"fetal adrenal tissue"	"fetal adrenal tissue"	"fetal adrenal tissue"	"fetal adrenal tissue"	"fetal adrenal tissue"	"neuroblastoma tissue"	"neuroblastoma tissue"	"neuroblastoma tissue"	"neuroblastoma tissue"	"neuroblastoma tissue"	"neuroblastoma tissue"
!Sample_organism_ch1	"Homo sapiens"	"Homo sapiens"	"Homo sapiens"	"Homo sapiens"	"Homo sapiens"	"Homo sapiens"	"Homo sapiens"	"Homo sapiens"	"Homo sapiens"	"Homo sapiens"	"Homo sapiens"	"Homo sapiens"	"Homo sapiens"	"Homo sapiens"	"Homo sapiens"	"Homo sapiens"	"Homo sapiens"	"Homo sapiens"	"Homo sapiens"	"Homo sapiens"	"Homo sapiens"	"Homo sapiens"
!Sample_characteristics_ch1	"Sex: male"	"Sex: female"	"Sex: male"	"Sex: female"	"Sex: male"	"Sex: female"	"Sex: male"	"Sex: male"	"Sex: male"	"Sex: female"	"age: 8+6 PCW (post-conception wk)"	"age: 8+3 PCW (post-conception wk)"	"age: 14+4 PCW (post-conception wk)"	"age: 13+2 PCW (post-conception wk)"	"age: about 4 PCW"	"age: about 4 PCW"	"Sex: male"	"Sex: male"	"Sex: male"	"Sex: female"	"Sex: female"	"Sex: male"
!Sample_characteristics_ch1	"age: 3Y3M"	"age: 2Y2M"	"age: 3Y4M"	"age: 3Y2M"	"age: 2M26D"	"age: 2M6D"	"age: 2Y5M"	"age: 5D"	"age: 9M10D"	"age: 3Y5M"	""	""	""	""	""	""	"age: 1Y11M"	"age: 23D"	"age: 3M23D"	"age: 2Y6M"	"age: 3M28D"	"age: 4Y8M"
!Sample_molecule_ch1	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"	"total RNA"
!Sample_extract_protocol_ch1	"Tumor tissues were resected by surgery and transported in DMEM/F12 medium on ice to the laboratory within an hour. The fetal adrenal tissues were collected  and transported in DMEM/F12 medium on ice to the laboratory within an hour."	"Tumor tissues were resected by surgery and transported in DMEM/F12 medium on ice to the laboratory within an hour. The fetal adrenal tissues were collected  and transported in DMEM/F12 medium on ice to the laboratory within an hour."	"Tumor tissues were resected by surgery and transported in DMEM/F12 medium on ice to the laboratory within an hour. The fetal adrenal tissues were collected  and transported in DMEM/F12 medium on ice to the laboratory within an hour."	"Tumor tissues were resected by surgery and transported in DMEM/F12 medium on ice to the laboratory within an hour. The fetal adrenal tissues were collected  and transported in DMEM/F12 medium on ice to the laboratory within an hour."	"Tumor tissues were resected by surgery and transported in DMEM/F12 medium on ice to the laboratory within an hour. The fetal adrenal tissues were collected  and transported in DMEM/F12 medium on ice to the laboratory within an hour."	"Tumor tissues were resected by surgery and transported in DMEM/F12 medium on ice to the laboratory within an hour. The fetal adrenal tissues were collected  and transported in DMEM/F12 medium on ice to the laboratory within an hour."	"Tumor tissues were resected by surgery and transported in DMEM/F12 medium on ice to the laboratory within an hour. The fetal adrenal tissues were collected  and transported in DMEM/F12 medium on ice to the laboratory within an hour."	"Tumor tissues were resected by surgery and transported in DMEM/F12 medium on ice to the laboratory within an hour. The fetal adrenal tissues were collected  and transported in DMEM/F12 medium on ice to the laboratory within an hour."	"Tumor tissues were resected by surgery and transported in DMEM/F12 medium on ice to the laboratory within an hour. The fetal adrenal tissues were collected  and transported in DMEM/F12 medium on ice to the laboratory within an hour."	"Tumor tissues were resected by surgery and transported in DMEM/F12 medium on ice to the laboratory within an hour. The fetal adrenal tissues were collected  and transported in DMEM/F12 medium on ice to the laboratory within an hour."	"Tumor tissues were resected by surgery and transported in DMEM/F12 medium on ice to the laboratory within an hour. The fetal adrenal tissues were collected  and transported in DMEM/F12 medium on ice to the laboratory within an hour."	"Tumor tissues were resected by surgery and transported in DMEM/F12 medium on ice to the laboratory within an hour. The fetal adrenal tissues were collected  and transported in DMEM/F12 medium on ice to the laboratory within an hour."	"Tumor tissues were resected by surgery and transported in DMEM/F12 medium on ice to the laboratory within an hour. The fetal adrenal tissues were collected  and transported in DMEM/F12 medium on ice to the laboratory within an hour."	"Tumor tissues were resected by surgery and transported in DMEM/F12 medium on ice to the laboratory within an hour. The fetal adrenal tissues were collected  and transported in DMEM/F12 medium on ice to the laboratory within an hour."	"Tumor tissues were resected by surgery and transported in DMEM/F12 medium on ice to the laboratory within an hour. The fetal adrenal tissues were collected  and transported in DMEM/F12 medium on ice to the laboratory within an hour."	"Tumor tissues were resected by surgery and transported in DMEM/F12 medium on ice to the laboratory within an hour. The fetal adrenal tissues were collected  and transported in DMEM/F12 medium on ice to the laboratory within an hour."	"Tumor tissues were resected by surgery and transported in DMEM/F12 medium on ice to the laboratory within an hour. The fetal adrenal tissues were collected  and transported in DMEM/F12 medium on ice to the laboratory within an hour."	"Tumor tissues were resected by surgery and transported in DMEM/F12 medium on ice to the laboratory within an hour. The fetal adrenal tissues were collected  and transported in DMEM/F12 medium on ice to the laboratory within an hour."	"Tumor tissues were resected by surgery and transported in DMEM/F12 medium on ice to the laboratory within an hour. The fetal adrenal tissues were collected  and transported in DMEM/F12 medium on ice to the laboratory within an hour."	"Tumor tissues were resected by surgery and transported in DMEM/F12 medium on ice to the laboratory within an hour. The fetal adrenal tissues were collected  and transported in DMEM/F12 medium on ice to the laboratory within an hour."	"Tumor tissues were resected by surgery and transported in DMEM/F12 medium on ice to the laboratory within an hour. The fetal adrenal tissues were collected  and transported in DMEM/F12 medium on ice to the laboratory within an hour."	"Tumor tissues were resected by surgery and transported in DMEM/F12 medium on ice to the laboratory within an hour. The fetal adrenal tissues were collected  and transported in DMEM/F12 medium on ice to the laboratory within an hour."
!Sample_extract_protocol_ch1	"For scRNA-seq, the tissues were dissociated into single cells suspensions using digestion buffer (DMEM/F12 Medium+ 2 mg/ml of collagenase IV + 100 IU/ml DNase I), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture’s protocol."	"For scRNA-seq, the tissues were dissociated into single cells suspensions using digestion buffer (DMEM/F12 Medium+ 2 mg/ml of collagenase IV + 100 IU/ml DNase I), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture’s protocol."	"For scRNA-seq, the tissues were dissociated into single cells suspensions using digestion buffer (DMEM/F12 Medium+ 2 mg/ml of collagenase IV + 100 IU/ml DNase I), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture’s protocol."	"For scRNA-seq, the tissues were dissociated into single cells suspensions using digestion buffer (DMEM/F12 Medium+ 2 mg/ml of collagenase IV + 100 IU/ml DNase I), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture’s protocol."	"For scRNA-seq, the tissues were dissociated into single cells suspensions using digestion buffer (DMEM/F12 Medium+ 2 mg/ml of collagenase IV + 100 IU/ml DNase I), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture’s protocol."	"For scRNA-seq, the tissues were dissociated into single cells suspensions using digestion buffer (DMEM/F12 Medium+ 2 mg/ml of collagenase IV + 100 IU/ml DNase I), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture’s protocol."	"For scRNA-seq, the tissues were dissociated into single cells suspensions using digestion buffer (DMEM/F12 Medium+ 2 mg/ml of collagenase IV + 100 IU/ml DNase I), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture’s protocol."	"For scRNA-seq, the tissues were dissociated into single cells suspensions using digestion buffer (DMEM/F12 Medium+ 2 mg/ml of collagenase IV + 100 IU/ml DNase I), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture’s protocol."	"For scRNA-seq, the tissues were dissociated into single cells suspensions using digestion buffer (DMEM/F12 Medium+ 2 mg/ml of collagenase IV + 100 IU/ml DNase I), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture’s protocol."	"For scRNA-seq, the tissues were dissociated into single cells suspensions using digestion buffer (DMEM/F12 Medium+ 2 mg/ml of collagenase IV + 100 IU/ml DNase I), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture’s protocol."	"For scRNA-seq, the tissues were dissociated into single cells suspensions using digestion buffer (DMEM/F12 Medium+ 2 mg/ml of collagenase IV + 100 IU/ml DNase I), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture’s protocol."	"For scRNA-seq, the tissues were dissociated into single cells suspensions using digestion buffer (DMEM/F12 Medium+ 2 mg/ml of collagenase IV + 100 IU/ml DNase I), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture’s protocol."	"For scRNA-seq, the tissues were dissociated into single cells suspensions using digestion buffer (DMEM/F12 Medium+ 2 mg/ml of collagenase IV + 100 IU/ml DNase I), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture’s protocol."	"For scRNA-seq, the tissues were dissociated into single cells suspensions using digestion buffer (DMEM/F12 Medium+ 2 mg/ml of collagenase IV + 100 IU/ml DNase I), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture’s protocol."	"For scRNA-seq, the tissues were dissociated into single cells suspensions using digestion buffer (DMEM/F12 Medium+ 2 mg/ml of collagenase IV + 100 IU/ml DNase I), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture’s protocol."	"For scRNA-seq, the tissues were dissociated into single cells suspensions using digestion buffer (DMEM/F12 Medium+ 2 mg/ml of collagenase IV + 100 IU/ml DNase I), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture’s protocol."	"For scRNA-seq, the tissues were dissociated into single cells suspensions using digestion buffer (DMEM/F12 Medium+ 2 mg/ml of collagenase IV + 100 IU/ml DNase I), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture’s protocol."	"For scRNA-seq, the tissues were dissociated into single cells suspensions using digestion buffer (DMEM/F12 Medium+ 2 mg/ml of collagenase IV + 100 IU/ml DNase I), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture’s protocol."	"For scRNA-seq, the tissues were dissociated into single cells suspensions using digestion buffer (DMEM/F12 Medium+ 2 mg/ml of collagenase IV + 100 IU/ml DNase I), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture’s protocol."	"For scRNA-seq, the tissues were dissociated into single cells suspensions using digestion buffer (DMEM/F12 Medium+ 2 mg/ml of collagenase IV + 100 IU/ml DNase I), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture’s protocol."	"For scRNA-seq, the tissues were dissociated into single cells suspensions using digestion buffer (DMEM/F12 Medium+ 2 mg/ml of collagenase IV + 100 IU/ml DNase I), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture’s protocol."	"For scRNA-seq, the tissues were dissociated into single cells suspensions using digestion buffer (DMEM/F12 Medium+ 2 mg/ml of collagenase IV + 100 IU/ml DNase I), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture’s protocol."
!Sample_taxid_ch1	"9606"	"9606"	"9606"	"9606"	"9606"	"9606"	"9606"	"9606"	"9606"	"9606"	"9606"	"9606"	"9606"	"9606"	"9606"	"9606"	"9606"	"9606"	"9606"	"9606"	"9606"	"9606"
!Sample_description	""	""	""	""	""	""	""	""	""	""	""	""	""	""	"F336_S8"	"F379_S7"	"S162T_S15"	"T175_S1"	"T188_S4"	"T200_S24"	"S214T_S22"	"T230_S5"
!Sample_data_processing	"CellRanger was used to base-calling, alignment, filtering and generation of normalized abundance measurements."	"CellRanger was used to base-calling, alignment, filtering and generation of normalized abundance measurements."	"CellRanger was used to base-calling, alignment, filtering and generation of normalized abundance measurements."	"CellRanger was used to base-calling, alignment, filtering and generation of normalized abundance measurements."	"CellRanger was used to base-calling, alignment, filtering and generation of normalized abundance measurements."	"CellRanger was used to base-calling, alignment, filtering and generation of normalized abundance measurements."	"CellRanger was used to base-calling, alignment, filtering and generation of normalized abundance measurements."	"CellRanger was used to base-calling, alignment, filtering and generation of normalized abundance measurements."	"CellRanger was used to base-calling, alignment, filtering and generation of normalized abundance measurements."	"CellRanger was used to base-calling, alignment, filtering and generation of normalized abundance measurements."	"CellRanger was used to base-calling, alignment, filtering and generation of normalized abundance measurements."	"CellRanger was used to base-calling, alignment, filtering and generation of normalized abundance measurements."	"CellRanger was used to base-calling, alignment, filtering and generation of normalized abundance measurements."	"CellRanger was used to base-calling, alignment, filtering and generation of normalized abundance measurements."	"CellRanger was used to base-calling, alignment, filtering and generation of normalized abundance measurements."	"CellRanger was used to base-calling, alignment, filtering and generation of normalized abundance measurements."	"CellRanger was used to base-calling, alignment, filtering and generation of normalized abundance measurements."	"CellRanger was used to base-calling, alignment, filtering and generation of normalized abundance measurements."	"CellRanger was used to base-calling, alignment, filtering and generation of normalized abundance measurements."	"CellRanger was used to base-calling, alignment, filtering and generation of normalized abundance measurements."	"CellRanger was used to base-calling, alignment, filtering and generation of normalized abundance measurements."	"CellRanger was used to base-calling, alignment, filtering and generation of normalized abundance measurements."
!Sample_data_processing	"Read 1: contains the 16bp 10x barcode and the 10bp UMI (bases 17-26).Index contains the sample barcode. Read 2 contains the cDNA information"	"Read 1: contains the 16bp 10x barcode and the 10bp UMI (bases 17-26).Index contains the sample barcode. Read 2 contains the cDNA information"	"Read 1: contains the 16bp 10x barcode and the 10bp UMI (bases 17-26).Index contains the sample barcode. Read 2 contains the cDNA information"	"Read 1: contains the 16bp 10x barcode and the 10bp UMI (bases 17-26).Index contains the sample barcode. Read 2 contains the cDNA information"	"Read 1: contains the 16bp 10x barcode and the 10bp UMI (bases 17-26).Index contains the sample barcode. Read 2 contains the cDNA information"	"Read 1: contains the 16bp 10x barcode and the 10bp UMI (bases 17-26).Index contains the sample barcode. Read 2 contains the cDNA information"	"Read 1: contains the 16bp 10x barcode and the 10bp UMI (bases 17-26).Index contains the sample barcode. Read 2 contains the cDNA information"	"Read 1: contains the 16bp 10x barcode and the 10bp UMI (bases 17-26).Index contains the sample barcode. Read 2 contains the cDNA information"	"Read 1: contains the 16bp 10x barcode and the 10bp UMI (bases 17-26).Index contains the sample barcode. Read 2 contains the cDNA information"	"Read 1: contains the 16bp 10x barcode and the 10bp UMI (bases 17-26).Index contains the sample barcode. Read 2 contains the cDNA information"	"Read 1: contains the 16bp 10x barcode and the 10bp UMI (bases 17-26).Index contains the sample barcode. Read 2 contains the cDNA information"	"Read 1: contains the 16bp 10x barcode and the 10bp UMI (bases 17-26).Index contains the sample barcode. Read 2 contains the cDNA information"	"Read 1: contains the 16bp 10x barcode and the 10bp UMI (bases 17-26).Index contains the sample barcode. Read 2 contains the cDNA information"	"Read 1: contains the 16bp 10x barcode and the 10bp UMI (bases 17-26).Index contains the sample barcode. Read 2 contains the cDNA information"	"Read 1: contains the 16bp 10x barcode and the 10bp UMI (bases 17-26).Index contains the sample barcode. Read 2 contains the cDNA information"	"Read 1: contains the 16bp 10x barcode and the 10bp UMI (bases 17-26).Index contains the sample barcode. Read 2 contains the cDNA information"	"Read 1: contains the 16bp 10x barcode and the 10bp UMI (bases 17-26).Index contains the sample barcode. Read 2 contains the cDNA information"	"Read 1: contains the 16bp 10x barcode and the 10bp UMI (bases 17-26).Index contains the sample barcode. Read 2 contains the cDNA information"	"Read 1: contains the 16bp 10x barcode and the 10bp UMI (bases 17-26).Index contains the sample barcode. Read 2 contains the cDNA information"	"Read 1: contains the 16bp 10x barcode and the 10bp UMI (bases 17-26).Index contains the sample barcode. Read 2 contains the cDNA information"	"Read 1: contains the 16bp 10x barcode and the 10bp UMI (bases 17-26).Index contains the sample barcode. Read 2 contains the cDNA information"	"Read 1: contains the 16bp 10x barcode and the 10bp UMI (bases 17-26).Index contains the sample barcode. Read 2 contains the cDNA information"
!Sample_data_processing	"Genome_build: GRCh38"	"Genome_build: GRCh38"	"Genome_build: GRCh38"	"Genome_build: GRCh38"	"Genome_build: GRCh38"	"Genome_build: GRCh38"	"Genome_build: GRCh38"	"Genome_build: GRCh38"	"Genome_build: GRCh38"	"Genome_build: GRCh38"	"Genome_build: GRCh38"	"Genome_build: GRCh38"	"Genome_build: GRCh38"	"Genome_build: GRCh38"	"Genome_build: GRCh38"	"Genome_build: GRCh38"	"Genome_build: GRCh38"	"Genome_build: GRCh38"	"Genome_build: GRCh38"	"Genome_build: GRCh38"	"Genome_build: GRCh38"	"Genome_build: GRCh38"
!Sample_data_processing	"Supplementary_files_format_and_content: tab-delimited text files include normalized median scaling values for each cell"	"Supplementary_files_format_and_content: tab-delimited text files include normalized median scaling values for each cell"	"Supplementary_files_format_and_content: tab-delimited text files include normalized median scaling values for each cell"	"Supplementary_files_format_and_content: tab-delimited text files include normalized median scaling values for each cell"	"Supplementary_files_format_and_content: tab-delimited text files include normalized median scaling values for each cell"	"Supplementary_files_format_and_content: tab-delimited text files include normalized median scaling values for each cell"	"Supplementary_files_format_and_content: tab-delimited text files include normalized median scaling values for each cell"	"Supplementary_files_format_and_content: tab-delimited text files include normalized median scaling values for each cell"	"Supplementary_files_format_and_content: tab-delimited text files include normalized median scaling values for each cell"	"Supplementary_files_format_and_content: tab-delimited text files include normalized median scaling values for each cell"	"Supplementary_files_format_and_content: tab-delimited text files include normalized median scaling values for each cell"	"Supplementary_files_format_and_content: tab-delimited text files include normalized median scaling values for each cell"	"Supplementary_files_format_and_content: tab-delimited text files include normalized median scaling values for each cell"	"Supplementary_files_format_and_content: tab-delimited text files include normalized median scaling values for each cell"	"Supplementary_files_format_and_content: tab-delimited text files include normalized median scaling values for each cell"	"Supplementary_files_format_and_content: tab-delimited text files include normalized median scaling values for each cell"	"Supplementary_files_format_and_content: tab-delimited text files include normalized median scaling values for each cell"	"Supplementary_files_format_and_content: tab-delimited text files include normalized median scaling values for each cell"	"Supplementary_files_format_and_content: tab-delimited text files include normalized median scaling values for each cell"	"Supplementary_files_format_and_content: tab-delimited text files include normalized median scaling values for each cell"	"Supplementary_files_format_and_content: tab-delimited text files include normalized median scaling values for each cell"	"Supplementary_files_format_and_content: tab-delimited text files include normalized median scaling values for each cell"
!Sample_platform_id	"GPL24676"	"GPL24676"	"GPL24676"	"GPL24676"	"GPL24676"	"GPL24676"	"GPL24676"	"GPL24676"	"GPL24676"	"GPL24676"	"GPL24676"	"GPL24676"	"GPL24676"	"GPL24676"	"GPL24676"	"GPL24676"	"GPL24676"	"GPL24676"	"GPL24676"	"GPL24676"	"GPL24676"	"GPL24676"
!Sample_contact_name	"rui,,Dong"	"rui,,Dong"	"rui,,Dong"	"rui,,Dong"	"rui,,Dong"	"rui,,Dong"	"rui,,Dong"	"rui,,Dong"	"rui,,Dong"	"rui,,Dong"	"rui,,Dong"	"rui,,Dong"	"rui,,Dong"	"rui,,Dong"	"rui,,Dong"	"rui,,Dong"	"rui,,Dong"	"rui,,Dong"	"rui,,Dong"	"rui,,Dong"	"rui,,Dong"	"rui,,Dong"
!Sample_contact_email	"rdong@fudan.edu.cn"	"rdong@fudan.edu.cn"	"rdong@fudan.edu.cn"	"rdong@fudan.edu.cn"	"rdong@fudan.edu.cn"	"rdong@fudan.edu.cn"	"rdong@fudan.edu.cn"	"rdong@fudan.edu.cn"	"rdong@fudan.edu.cn"	"rdong@fudan.edu.cn"	"rdong@fudan.edu.cn"	"rdong@fudan.edu.cn"	"rdong@fudan.edu.cn"	"rdong@fudan.edu.cn"	"rdong@fudan.edu.cn"	"rdong@fudan.edu.cn"	"rdong@fudan.edu.cn"	"rdong@fudan.edu.cn"	"rdong@fudan.edu.cn"	"rdong@fudan.edu.cn"	"rdong@fudan.edu.cn"	"rdong@fudan.edu.cn"
!Sample_contact_institute	"Department of Pediatric Surgery, Children’s Hospital of Fudan University, and Shanghai Key Laboratory of Birth Defect"	"Department of Pediatric Surgery, Children’s Hospital of Fudan University, and Shanghai Key Laboratory of Birth Defect"	"Department of Pediatric Surgery, Children’s Hospital of Fudan University, and Shanghai Key Laboratory of Birth Defect"	"Department of Pediatric Surgery, Children’s Hospital of Fudan University, and Shanghai Key Laboratory of Birth Defect"	"Department of Pediatric Surgery, Children’s Hospital of Fudan University, and Shanghai Key Laboratory of Birth Defect"	"Department of Pediatric Surgery, Children’s Hospital of Fudan University, and Shanghai Key Laboratory of Birth Defect"	"Department of Pediatric Surgery, Children’s Hospital of Fudan University, and Shanghai Key Laboratory of Birth Defect"	"Department of Pediatric Surgery, Children’s Hospital of Fudan University, and Shanghai Key Laboratory of Birth Defect"	"Department of Pediatric Surgery, Children’s Hospital of Fudan University, and Shanghai Key Laboratory of Birth Defect"	"Department of Pediatric Surgery, Children’s Hospital of Fudan University, and Shanghai Key Laboratory of Birth Defect"	"Department of Pediatric Surgery, Children’s Hospital of Fudan University, and Shanghai Key Laboratory of Birth Defect"	"Department of Pediatric Surgery, Children’s Hospital of Fudan University, and Shanghai Key Laboratory of Birth Defect"	"Department of Pediatric Surgery, Children’s Hospital of Fudan University, and Shanghai Key Laboratory of Birth Defect"	"Department of Pediatric Surgery, Children’s Hospital of Fudan University, and Shanghai Key Laboratory of Birth Defect"	"Department of Pediatric Surgery, Children’s Hospital of Fudan University, and Shanghai Key Laboratory of Birth Defect"	"Department of Pediatric Surgery, Children’s Hospital of Fudan University, and Shanghai Key Laboratory of Birth Defect"	"Department of Pediatric Surgery, Children’s Hospital of Fudan University, and Shanghai Key Laboratory of Birth Defect"	"Department of Pediatric Surgery, Children’s Hospital of Fudan University, and Shanghai Key Laboratory of Birth Defect"	"Department of Pediatric Surgery, Children’s Hospital of Fudan University, and Shanghai Key Laboratory of Birth Defect"	"Department of Pediatric Surgery, Children’s Hospital of Fudan University, and Shanghai Key Laboratory of Birth Defect"	"Department of Pediatric Surgery, Children’s Hospital of Fudan University, and Shanghai Key Laboratory of Birth Defect"	"Department of Pediatric Surgery, Children’s Hospital of Fudan University, and Shanghai Key Laboratory of Birth Defect"
!Sample_contact_address	"399 Wan Yuan Road"	"399 Wan Yuan Road"	"399 Wan Yuan Road"	"399 Wan Yuan Road"	"399 Wan Yuan Road"	"399 Wan Yuan Road"	"399 Wan Yuan Road"	"399 Wan Yuan Road"	"399 Wan Yuan Road"	"399 Wan Yuan Road"	"399 Wan Yuan Road"	"399 Wan Yuan Road"	"399 Wan Yuan Road"	"399 Wan Yuan Road"	"399 Wan Yuan Road"	"399 Wan Yuan Road"	"399 Wan Yuan Road"	"399 Wan Yuan Road"	"399 Wan Yuan Road"	"399 Wan Yuan Road"	"399 Wan Yuan Road"	"399 Wan Yuan Road"
!Sample_contact_city	"Shanghai"	"Shanghai"	"Shanghai"	"Shanghai"	"Shanghai"	"Shanghai"	"Shanghai"	"Shanghai"	"Shanghai"	"Shanghai"	"Shanghai"	"Shanghai"	"Shanghai"	"Shanghai"	"Shanghai"	"Shanghai"	"Shanghai"	"Shanghai"	"Shanghai"	"Shanghai"	"Shanghai"	"Shanghai"
!Sample_contact_zip/postal_code	"201102"	"201102"	"201102"	"201102"	"201102"	"201102"	"201102"	"201102"	"201102"	"201102"	"201102"	"201102"	"201102"	"201102"	"201102"	"201102"	"201102"	"201102"	"201102"	"201102"	"201102"	"201102"
!Sample_contact_country	"China"	"China"	"China"	"China"	"China"	"China"	"China"	"China"	"China"	"China"	"China"	"China"	"China"	"China"	"China"	"China"	"China"	"China"	"China"	"China"	"China"	"China"
!Sample_data_row_count	"0"	"0"	"0"	"0"	"0"	"0"	"0"	"0"	"0"	"0"	"0"	"0"	"0"	"0"	"0"	"0"	"0"	"0"	"0"	"0"	"0"	"0"
!Sample_instrument_model	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"	"Illumina NovaSeq 6000"
!Sample_library_selection	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"	"cDNA"
!Sample_library_source	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"	"transcriptomic"
!Sample_library_strategy	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"	"RNA-Seq"
!Sample_relation	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN12799275"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN12799274"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN12799273"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN12799272"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN12799270"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN12799269"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN12799268"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN12799266"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN12799264"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN12799263"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN12799261"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN12799259"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN12799258"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN12799257"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN15453062"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN15453069"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN15453068"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN15453067"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN15453066"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN15453065"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN15453064"	"BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN15453063"
!Sample_relation	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX6881705"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX6881706"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX6881707"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX6881708"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX6881709"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX6881710"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX6881711"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX6881712"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX6881713"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX6881714"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX6881716"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX6881717"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX6881718"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX6881719"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX8668244"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX8668245"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX8668246"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX8668247"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX8668248"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX8668249"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX8668250"	"SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX8668251"
!Sample_supplementary_file_1	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4088nnn/GSM4088774/suppl/GSM4088774_T10_gene_cell_exprs_table.xls.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4088nnn/GSM4088775/suppl/GSM4088775_T19_gene_cell_exprs_table.xls.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4088nnn/GSM4088776/suppl/GSM4088776_T27_gene_cell_exprs_table.xls.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4088nnn/GSM4088777/suppl/GSM4088777_T34_gene_cell_exprs_table.xls.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4088nnn/GSM4088778/suppl/GSM4088778_T40_gene_cell_exprs_table.xls.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4088nnn/GSM4088779/suppl/GSM4088779_T44_gene_cell_exprs_table.xls.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4088nnn/GSM4088780/suppl/GSM4088780_T69_gene_cell_exprs_table.xls.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4088nnn/GSM4088781/suppl/GSM4088781_T71_gene_cell_exprs_table.xls.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4088nnn/GSM4088782/suppl/GSM4088782_T75_gene_cell_exprs_table.xls.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4088nnn/GSM4088783/suppl/GSM4088783_T92_gene_cell_exprs_table.xls.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4088nnn/GSM4088785/suppl/GSM4088785_F2_gene_cell_exprs_table.xls.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4088nnn/GSM4088786/suppl/GSM4088786_F7_gene_cell_exprs_table.xls.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4088nnn/GSM4088787/suppl/GSM4088787_F106_gene_cell_exprs_table.xls.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4088nnn/GSM4088788/suppl/GSM4088788_F107_gene_cell_exprs_table.xls.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4654nnn/GSM4654667/suppl/GSM4654667_F366_barcodes.tsv.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4654nnn/GSM4654668/suppl/GSM4654668_F379_barcodes.tsv.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4654nnn/GSM4654669/suppl/GSM4654669_T162_barcodes.tsv.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4654nnn/GSM4654670/suppl/GSM4654670_T175_barcodes.tsv.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4654nnn/GSM4654671/suppl/GSM4654671_T188_barcodes.tsv.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4654nnn/GSM4654672/suppl/GSM4654672_T200_barcodes.tsv.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4654nnn/GSM4654673/suppl/GSM4654673_T214_barcodes.tsv.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4654nnn/GSM4654674/suppl/GSM4654674_T230_barcodes.tsv.gz"
!Sample_supplementary_file_2	""	""	""	""	""	""	""	""	""	""	""	""	""	""	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4654nnn/GSM4654667/suppl/GSM4654667_F366_features.tsv.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4654nnn/GSM4654668/suppl/GSM4654668_F379_features.tsv.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4654nnn/GSM4654669/suppl/GSM4654669_T162_features.tsv.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4654nnn/GSM4654670/suppl/GSM4654670_T175_features.tsv.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4654nnn/GSM4654671/suppl/GSM4654671_T188_features.tsv.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4654nnn/GSM4654672/suppl/GSM4654672_T200_features.tsv.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4654nnn/GSM4654673/suppl/GSM4654673_T214_features.tsv.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4654nnn/GSM4654674/suppl/GSM4654674_T230_features.tsv.gz"
!Sample_supplementary_file_3	""	""	""	""	""	""	""	""	""	""	""	""	""	""	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4654nnn/GSM4654667/suppl/GSM4654667_F366_matrix.mtx.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4654nnn/GSM4654668/suppl/GSM4654668_F379_matrix.mtx.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4654nnn/GSM4654669/suppl/GSM4654669_T162_matrix.mtx.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4654nnn/GSM4654670/suppl/GSM4654670_T175_matrix.mtx.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4654nnn/GSM4654671/suppl/GSM4654671_T188_matrix.mtx.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4654nnn/GSM4654672/suppl/GSM4654672_T200_matrix.mtx.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4654nnn/GSM4654673/suppl/GSM4654673_T214_matrix.mtx.gz"	"ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM4654nnn/GSM4654674/suppl/GSM4654674_T230_matrix.mtx.gz"
!series_matrix_table_begin
"ID_REF"	"GSM4088774"	"GSM4088775"	"GSM4088776"	"GSM4088777"	"GSM4088778"	"GSM4088779"	"GSM4088780"	"GSM4088781"	"GSM4088782"	"GSM4088783"	"GSM4088785"	"GSM4088786"	"GSM4088787"	"GSM4088788"	"GSM4654667"	"GSM4654668"	"GSM4654669"	"GSM4654670"	"GSM4654671"	"GSM4654672"	"GSM4654673"	"GSM4654674"
!series_matrix_table_end